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1Department of Biomedical and Molecular Sciences,Queen’s University, 2National Institute of Neurological Disorders and Stroke,Porter Neuroscience Research Center, 3Research Institute of Genome-Based Biofactory,National Institute of Advanced Industrial Science and Technology, 4The Robert H. Smith Faculty of Agriculture, Food and Environment, Institute of Biochemistry, Food Science, and Nutrition,The Hebrew University of Jerusalem
Chapitres
- 00:05Titre
- 01:14Growing Single Ice Crystals
- 03:18Determining Singularity and Orientation o the Ice Crystal
- 04:37Absorption of Fluorescently Labeled Antifreeze Proteins to a Single Ice Crystal
- 07:20Results: Fluorescence-based Ice Plane Affinity Analysis Images
- 08:18Conclusion
Frostvæskeproteiner (AFPer) binder seg til bestemte isplan for å forhindre eller bremse isveksten. Fluorescensbasert isplanaffinitet (FIPA) analyse er en modifikasjon av den opprinnelige isetsingsmetoden for bestemmelse av AFP-bundet isplan. AFPer er fluorescerende merket, innlemmet i makroskopiske enkeltiskrystaller og visualisert under UV-lys.
