Journal
/
Bio-ingénierie
/
Stretching Micropatterned Cells on a PDMS Membrane
Journal JoVE
Bio-ingénierie
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Bio-ingénierie
Stretching Micropatterned Cells on a PDMS Membrane

Stretching Micropatterned Cells on a PDMS Membrane

DOI:
10.3791/51193-v

•

09:41 min

•

January 22, 2014

•

,
1UMR 144,Institut Curie

Chapitres

  • 00:05Titre
  • 01:30Passivation of the Polydimethylsiloxane (PDMS)
  • 03:58Patterning of the PDMS
  • 04:47Mounting the Device
  • 06:34Patterning the Cells and Stretching
  • 08:30Results: Phase Contrast Images of Micropatterned RPE-1 Cell on PDMS Surface
  • 09:20Conclusion

Summary

Traduction automatique

Traduction automatique

This manuscript presents a technique to apply or release forces on adherent cells or tissues using unidirectional stretching.

Tags

Micropatterned CellsPDMS MembraneMechanical StretchingCell MechanicsCell Response To StressDeep UV PatterningMicrometric ScrewBiocompatible Chamber

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/51193?article_type=v

Micropatterned Surfaces to Study Hyaluronic Acid Interactions with Cancer Cells
check_url/fr/51193?article_type=v

Improved Visualization and Quantitative Analysis of Drug Effects Using Micropatterned Cells
check_url/fr/51193?article_type=v

Fabrication of Micropatterned Hydrogels for Neural Culture Systems using Dynamic Mask Projection Photolithography
check_url/fr/51193?article_type=v

Stretching Short Sequences of DNA with Constant Force Axial Optical Tweezers
check_url/fr/51193?article_type=v

A Novel Stretching Platform for Applications in Cell and Tissue Mechanobiology
check_url/fr/51193?article_type=v

Luminescence Resonance Energy Transfer to Study Conformational Changes in Membrane Proteins Expressed in Mammalian Cells
check_url/fr/51193?article_type=v

Optimizing Attachment of Human Mesenchymal Stem Cells on Poly(ε-caprolactone) Electrospun Yarns
check_url/fr/51193?article_type=v

Gene Transfection toward Spheroid Cells on Micropatterned Culture Plates for Genetically-modified Cell Transplantation
check_url/fr/51193?article_type=v

Microfluidic Genipin Deposition Technique for Extended Culture of Micropatterned Vascular Muscular Thin Films
check_url/fr/51193?article_type=v

Double Emulsion Generation Using a Polydimethylsiloxane (PDMS) Co-axial Flow Focus Device

Read Article