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FIBS功能的无创性代谢轮廓

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Bio-ingénierie

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Journal JoVE Bio-ingénierie

FIBS-enabled Noninvasive Metabolic Profiling

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FIBS功能的无创性代谢轮廓

DOI:

10.3791/51200-v

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09:16 min

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February 03, 2014

•

Alireza Behjousiar², Antony Constantinou, Karen M. Polizzi, Cleo Kontoravdi

¹Centre for Process Systems Engineering, Department of Chemical Engineering and Chemical Technology,Imperial College London, ²Centre for Synthetic Biology and Innovation, Division of Molecular Biosciences,Imperial College London

Chapitres

00:05Titre
01:18Cell Line Revival and Maintenance
02:06Cell Line Transfection with the Biosensor Gene-containing Plasmid
03:47Batch and Fed-batch Cell growth Curve
04:44FRET Ratio Measurements
05:36Metabolite Assays
06:08Results: Representative Fed-batch Culture and Corresponding FRET Measurements
08:54Conclusion

Summary

Traduction automatique

如何在原位代谢轮廓校正共振能量转移集成生物传感器（FIBS）用于描述呈现。该FIBS可用于测量非侵入性代谢物辅助的代谢模式和生物过程条件的高通量筛选的发展的细胞内水平。

Tags

Keywords: FIBS Noninvasive Metabolic Profiling Computational Biology High-throughput Experimental Systems In Situ Monitoring Fluorescent Protein-based In Vivo Biosensors FRET FRET Integrated Biological Sensors CHO Cell Lines Glucose Glutamine Intracellular Metabolite Concentration Fed-batch Cell Culture

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