Journal
/
/
En cGMP-gældende Expansion Metode til aggregater af humane neurale Stem og stamceller arvet fra pluripotente stamceller eller fostrets hjerne Tissue
Journal JoVE
Neurosciences
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Neurosciences
A cGMP-applicable Expansion Method for Aggregates of Human Neural Stem and Progenitor Cells Derived From Pluripotent Stem Cells or Fetal Brain Tissue
DOI:

09:37 min

June 15, 2014

, ,

Chapitres

  • 00:05Titre
  • 01:36hNPC Observations
  • 02:13Chopper Setup
  • 03:22Pre-chop Procedure
  • 05:58Chop Procedure
  • 07:08Post-chop Procedure
  • 08:11Representative Results of hNPCs Aggregates Using a cGMP-applicable Expansion Method
  • 09:04Conclusion

Summary

Traduction automatique

Denne protokol beskriver en ny mekanisk sønderdeling, der tillader udvidelse af sfæriske neurale stamceller og stamcelle aggregater uden dissociation til en enkelt cellesuspension. Fastholdelse af celle / celle kontakt giver mulighed for hurtig og stabil vækst i over 40 passager.

Vidéos Connexes

Read Article