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一个鼠标,两种文化:隔离和成人神经的培养干细胞从单个小鼠的两个神经源性区
Journal JoVE
Neurosciences
This content is Free Access.
Journal JoVE Neurosciences
One Mouse, Two Cultures: Isolation and Culture of Adult Neural Stem Cells from the Two Neurogenic Zones of Individual Mice
DOI:

09:52 min

February 25, 2014

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Chapitres

  • 00:05Titre
  • 01:41Microdissection of Mouse Brain
  • 04:13SVZ Tissue Dissociation
  • 05:43DG Tissue Dissociation
  • 07:36Cell Plating for Monolayer and Neurosphere Culture Generation
  • 08:17Results: Isolation and Culture of Adult Neural Stem Cells from the Two Neurogenic Zones of Individual Mice
  • 09:21Conclusion

Summary

Traduction automatique

在这里,我们描述的同时产生神经前体细胞培养的一个详细的协议,因为无论是贴壁单层或神经球,从脑室下区和个体成年小鼠海马齿状回。

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