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Generering af Myospheres Fra hESCs ved Epigenetisk Omprogrammering
Journal JoVE
Biologie
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Journal JoVE Biologie
Generation of Myospheres From hESCs by Epigenetic Reprogramming

Generering af Myospheres Fra hESCs ved Epigenetisk Omprogrammering

DOI:
10.3791/51243-v

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09:32 min

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June 21, 2014

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1Muscle Development and Regeneration Program,Sanford-Burnham Institute for Medical Research, 2IRCCS Fondazione Santa Lucia

Chapitres

  • 00:05Titre
  • 01:23Infection Procedure
  • 03:46Culturing the Infected Cells
  • 05:35EB-like Induction and Differentiation
  • 07:33Representative Results: Myogenic Differentiation of Human Embryonic Stem Cells
  • 08:56Conclusion

Summary

Traduction automatique

Traduction automatique

Her beskriver vi en protokol baseret på epigenetiske omprogrammering af humane embryonale stamceller (hESCs) retning af at skabe en homogen population af skeletmuskulaturen stamfædre, at der under tolerante dyrkningsbetingelser danner tredimensionelle klynger af kontraktile myofibre (myospheres), som rekapitulere biologiske funktioner i menneskets skelet muskler.

Tags

HESCsSkeletal Muscle CellsMyospheresEpigenetic ReprogrammingMyoDBAF60CNeuromuscular Disorders3D Contractile StructuresStem Cell DifferentiationMiniaturized Skeletal Muscles

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