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1London Regional Cancer Program,London Health Sciences Centre, 2Department of Anatomy & Cell Biology, Schulich School of Medicine and Dentistry,Western University, 3Special Hematology/Flow Cytometry,London Health Sciences Centre, 4Lawson Health Research Institute, 5Department of Oncology,Western University
Chapitres
- 00:05Titre
- 01:42Human Blood Sample Preparation and Processing
- 03:25Sample Scanning on the Analysis Instrument
- 04:58CTC Characterization for User-defined Markers Using the CSS
- 06:08Adaptation of the Standard CSS Protocol: Preclinical Mouse Models
- 07:42CTC Staining
- 09:20Scanning of Manually Separated Samples
- 10:13Results: CTC Assays in Clinical and Preclinical Research
- 13:43Conclusion
Cirkulerende tumorceller (CTCs) er prognostisk i flere metastatiske cancere. Dette håndskrift beskriver guldstandarden CellSearch systemet (CSS) CTC tælling platform og fremhæver fælles misklassifikation fejl. Desuden er to tilpassede protokoller beskrevet for brugerdefineret markør karakterisering af CTCs og CTC optælling i prækliniske musemodeller af metastaser ved hjælp af denne teknologi.
