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1London Regional Cancer Program,London Health Sciences Centre, 2Department of Anatomy & Cell Biology, Schulich School of Medicine and Dentistry,Western University, 3Special Hematology/Flow Cytometry,London Health Sciences Centre, 4Lawson Health Research Institute, 5Department of Oncology,Western University
Chapitres
- 00:05Titre
- 01:42Human Blood Sample Preparation and Processing
- 03:25Sample Scanning on the Analysis Instrument
- 04:58CTC Characterization for User-defined Markers Using the CSS
- 06:08Adaptation of the Standard CSS Protocol: Preclinical Mouse Models
- 07:42CTC Staining
- 09:20Scanning of Manually Separated Samples
- 10:13Results: CTC Assays in Clinical and Preclinical Research
- 13:43Conclusion
Sirkulerende tumorceller (CTCs) er prognostisk i flere metastatisk kreft. Dette manuskriptet beskriver gullstandarden CellSearch system (CSS) CTC opplisting plattform og høydepunkter vanlige feilklassifisering feil. I tillegg er to tilpasset protokoller beskrevet for brukerdefinert markør karakterisering av CTCs og CTC oppregning i prekliniske musemodeller av metastasering ved hjelp av denne teknologien.
