Journal
/
Médecine
/
Sequential In vivo Imaging of Osteogenic Stem/Progenitor Cells During Fracture Repair
Journal JoVE
Médecine
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Médecine
Sequential In vivo Imaging of Osteogenic Stem/Progenitor Cells During Fracture Repair

Sequential In vivo Imaging of Osteogenic Stem/Progenitor Cells During Fracture Repair

DOI:
10.3791/51289-v

•

10:30 min

•

May 23, 2014

•

, , ,
1Center for Regenerative Medicine, Massachusetts General Hospital,Harvard Stem Cell Institute, 2Wellman Center for Photomedicine and Center for Systems Biology, Massachusetts General Hospital,Harvard Medical School

Chapitres

  • 00:05Titre
  • 01:57Microfracture Injury
  • 04:35Intravital Imaging
  • 07:51Results: Representative Osteogenic Stem and Progenitor Cell Imaging
  • 09:54Conclusion

Summary

Traduction automatique

Traduction automatique

Quantitative measurement of bone progenitor function in fracture healing requires high resolution serial imaging technology. Here, protocols are provided for using intravital microscopy and osteo-lineage tracking to sequentially image and quantify the migration, proliferation and differentiation of endogenous osteogenic stem/progenitor cells in the process of repairing bone fracture.

Tags

Bone RepairOsteogenic Stem/progenitor CellsIn Vivo ImagingCell TracingMicrofractureCalvarial BoneBone RegenerationStem Cell Regulation

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

In vivo Dual Substrate Bioluminescent Imaging

In vivo 19F MRI for Cell Tracking

In Vivo Dynamics of Retinal Microglial Activation During Neurodegeneration: Confocal Ophthalmoscopic Imaging and Cell Morphometry in Mouse Glaucoma

In Vivo Imaging and Tracking of Technetium-99m Labeled Bone Marrow Mesenchymal Stem Cells in Equine Tendinopathy

Studying Pancreatic Cancer Stem Cell Characteristics for Developing New Treatment Strategies

Depletion of Mouse Cells from Human Tumor Xenografts Significantly Improves Downstream Analysis of Target Cells

In Vivo Evaluation of Fracture Callus Development During Bone Healing in Mice Using an MRI-compatible Osteosynthesis Device for the Mouse Femur

Middle Cerebral Artery Occlusion Allowing Reperfusion via Common Carotid Artery Repair in Mice

Longitudinal In Vivo Imaging and Quantification of Human Pancreatic Islet Grafting and Contributing Host Cells in the Anterior Eye Chamber

A Simplified Stepwise Approach to Echo Guidance during Percutaneous Mitral Valve Repair

Read Article