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Preparation of DNA-crosslinked Polyacrylamide Hydrogels
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Journal JoVE Biologie
Preparation of DNA-crosslinked Polyacrylamide Hydrogels

Preparation of DNA-crosslinked Polyacrylamide Hydrogels

DOI:
10.3791/51323-v

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09:06 min

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August 27, 2014

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,
1New Jersey Neuroscience Institute,JFK Medical Center, 2Department of Biomedical Engineering,Rutgers University, 3Department of Mechanical and Aerospace Engineering,Rutgers University

Chapitres

  • 00:05Titre
  • 01:46Preparation of DNA Gels
  • 05:34Gel Immobilization on Glass
  • 06:34Gel Functionalization
  • 07:34Results: Analysis of Stiffening and Softening DNA-crosslinked Polyacrylamide Hydrogels
  • 08:33Conclusion

Summary

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Our laboratory has developed DNA-crosslinked polyacrylamide hydrogels, a dynamic hydrogel system, to better understand the effects of modulating tissue stiffness on cell function. Here, we provide schematics, descriptions, and protocols to prepare these hydrogels.

Tags

DNA-crosslinked Polyacrylamide HydrogelsMechanobiologyDynamic StiffnessDNA CrosslinksPolyacrylamide BackboneReversible ControlGel ElasticityFibroblastNeuron Behavior

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