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Zebrafish भ्रूण में oxidative तनाव का विश्लेषण
Journal JoVE
Biologie
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Journal JoVE Biologie
Analysis of Oxidative Stress in Zebrafish Embryos
DOI:

11:05 min

July 07, 2014

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Chapitres

  • 00:05Titre
  • 01:54Preparations for Oxidative Stress Induction and ROS-detection
  • 03:01Oxidative Stress Induction
  • 04:28Whole Mount ROS-detection
  • 05:56Single Cell ROS-detection
  • 09:08ROS-detection in Whole Mount and Single Cells
  • 10:30Conclusion

Summary

Traduction automatique

Here we report a protocol to measure oxidative stress in living zebrafish embryos. This procedure allows reactive oxygen species (ROS) detection in both whole embryo tissues and single-cell populations. This protocol will accomplish both qualitative and quantitative analyses.

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