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Immunologie et infection
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Påvisning av Fluorescent Nanoparticle Interaksjoner med Primær immun celle subpopulasjoner av flowcytometri
Journal JoVE
Immunologie et infection
This content is Free Access.
Journal JoVE Immunologie et infection
Detection of Fluorescent Nanoparticle Interactions with Primary Immune Cell Subpopulations by Flow Cytometry

Påvisning av Fluorescent Nanoparticle Interaksjoner med Primær immun celle subpopulasjoner av flowcytometri

DOI:
10.3791/51345-v

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07:31 min

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March 28, 2014

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, , , , , ,
1Center for Micro-BioRobotics @SSSA,Istituto Italiano di Tecnologia, 2Department of Biology,University of Pisa, 3Center for Biomolecular Nanotechnologies @UniLe,Istituto Italiano di Tecnologia

Chapitres

  • 00:05Titre
  • 00:56Nanoparticle Internalization in Blood Leukocytes and Purified Monocytes
  • 02:22Flow Cytometric Analysis
  • 03:44Results: Representative FITC-SiO2 Nanoparticle Internalization Analyses
  • 06:55Conclusion

Summary

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Analyse av nanopartikler interaksjon med definerte subpopulasjoner av immunceller av flowcytometri.

Tags

Flow CytometryNanoparticle InteractionImmune CellsFITC-SiO2 NanoparticlesRhodamine-SiO2 NanoparticlesPrimary MicrogliaGFP-expressing Monocyte/macrophage LineageCell FluorescenceSide-scattered Light

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