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Mesure simultanée du pH dans endocytose et cytosoliques compartiments dans les cellules vivantes en utilisant la microscopie confocale
Journal JoVE
Biologie
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Journal JoVE Biologie
Simultaneous pH Measurement in Endocytic and Cytosolic Compartments in Living Cells using Confocal Microscopy
DOI:

09:46 min

April 28, 2014

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Chapitres

  • 00:05Titre
  • 01:41Preparing Solutions and Cells
  • 03:58Imaging the Cells
  • 05:53Data Analysis
  • 07:03Intracellular pH Changes in HT-1080 Cells
  • 08:57Conclusion

Summary

Traduction automatique

Several methods are available for measuring intracellular pH but very few of these allow simultaneous measurement of cytoplasmic and organellar pH. Here, we describe in detail a rapid and accurate methodology to simultaneously measure cytoplasmic and vesicular pH by ratiometric imaging of living cells.

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