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最佳:条码启用四分体测序
Journal JoVE
Biologie
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Journal JoVE Biologie
BEST: Barcode Enabled Sequencing of Tetrads
DOI:

12:59 min

May 01, 2014

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Chapitres

  • 00:05Titre
  • 01:54Sporulating Library of Transformed Cells to Obtain Barcoded Tetrads
  • 03:29Establishing the FACS Gates for Tetrad Sorting
  • 06:14Sorting Tetrads Onto an Agar Plate
  • 08:16Separating Spores
  • 09:25Harvesting Colonies
  • 10:54Results: BEST is an Efficient Way to Isolate Spores from Tetrads
  • 12:37Conclusion

Summary

Traduction automatique

四分体启用条码序列(BEST)取代分离,破坏和间距四分体的手动流程。的最优隔离四分体通过荧光激活细胞分选到琼脂平板上,通过搅拌与玻璃珠分离出孢子,并确定哪个随机排列菌落,从同一原始四分使用分子条形码而得。

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