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RNAscope for In situ Detection of Transcriptionally Active Human Papillomavirus in Head and Neck Squamous Cell Carcinoma
Journal JoVE
Médecine
This content is Free Access.
Journal JoVE Médecine
RNAscope for In situ Detection of Transcriptionally Active Human Papillomavirus in Head and Neck Squamous Cell Carcinoma
DOI:

10:26 min

March 11, 2014

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Chapitres

  • 00:05Titre
  • 02:14Sample, Equipment, and Reagent Preparation
  • 04:07RNAscope Assay
  • 08:15Results: RNAscope Assay Detects HR-HPV in FFPE Tumor Tissue Sections
  • 09:45Conclusion

Summary

Traduction automatique

The presence of high-risk HPV in head and neck tumor tissue is associated with favorable outcomes. The recently developed RNA in situ hybridization technique called RNAscope allows direct visualization of HPV E6/E7 mRNA in FFPE tissue sections.

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