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Investigating Protein-protein Interactions in Live Cells Using Bioluminescence Resonance Energy Transfer
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Journal JoVE Biologie
Investigating Protein-protein Interactions in Live Cells Using Bioluminescence Resonance Energy Transfer

Investigating Protein-protein Interactions in Live Cells Using Bioluminescence Resonance Energy Transfer

DOI:
10.3791/51438-v

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11:46 min

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May 26, 2014

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, , ,
1Language and Genetics Department,Max Planck Institute for Psycholinguistics, 2Donders Institute for Brain, Cognition and Behaviour

Chapitres

  • 00:05Titre
  • 01:36Preparation of DNA Mixes
  • 04:25Transfection
  • 05:59Measurement of BRET Signal
  • 07:14Results: Assessing FOXP2 Protein-protein Interactions Using BRET
  • 11:09Conclusion

Summary

Traduction automatique

Traduction automatique

Interactions between proteins are fundamental to all cellular processes. Using Bioluminescence Resonance Energy Transfer, the interaction between a pair of proteins can be monitored in live cells and in real time. Furthermore, the effects of potentially pathogenic mutations can be assessed.

Tags

Bioluminescence Resonance Energy Transfer (BRET)Protein-protein InteractionsLive CellsRenilla Reniformis LuciferaseYellow Fluorescent Protein (YFP)Fusion ProteinsMammalian CellsYeast Two-hybridMass Spectrometry ProteomicsPost-translational ModificationsMutations

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