Journal
/
Biologie
/
Undersøgelse af protein-protein interaktioner i levende celler Brug Bioluminescens Resonance Energy Transfer
Journal JoVE
Biologie
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Biologie
Investigating Protein-protein Interactions in Live Cells Using Bioluminescence Resonance Energy Transfer

Undersøgelse af protein-protein interaktioner i levende celler Brug Bioluminescens Resonance Energy Transfer

DOI:
10.3791/51438-v

•

11:46 min

•

May 26, 2014

•

, , ,
1Language and Genetics Department,Max Planck Institute for Psycholinguistics, 2Donders Institute for Brain, Cognition and Behaviour

Chapitres

  • 00:05Titre
  • 01:36Preparation of DNA Mixes
  • 04:25Transfection
  • 05:59Measurement of BRET Signal
  • 07:14Results: Assessing FOXP2 Protein-protein Interactions Using BRET
  • 11:09Conclusion

Summary

Traduction automatique

Traduction automatique

Interaktioner mellem proteiner er grundlæggende for alle cellulære processer. Brug Bioluminescens Resonance Energy Transfer, kan overvåges interaktionen mellem et par af proteiner i levende celler og i realtid. Endvidere kan virkningerne af potentielt patogene mutationer vurderes.

Tags

Bioluminescence Resonance Energy Transfer (BRET)Protein-protein InteractionsLive CellsRenilla Reniformis LuciferaseYellow Fluorescent Protein (YFP)Fusion ProteinsMammalian CellsYeast Two-hybridMass Spectrometry ProteomicsPost-translational ModificationsMutations

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

Microfluidic Co-culture of Epithelial Cells and Bacteria for Investigating Soluble Signal-mediated Interactions

Investigating Tissue- and Organ-specific Phytochrome Responses using FACS-assisted Cell-type Specific Expression Profiling in Arabidopsis thaliana

Bimolecular Fluorescence Complementation (BiFC) Assay for Protein-Protein Interaction in Onion Cells Using the Helios Gene Gun

In-vivo Detection of Protein-protein Interactions on Micro-patterned Surfaces

Imaging Protein-protein Interactions in vivo

Real-time overvågning af ligand-receptor interaktioner med Fluorescence Resonance Energy Transfer

Protein-protein interaktioner visualiseret ved bimolekylær Fluorescens komplementering i tobaksprotoplaster og blade

Sondering High-density funktionelt protein Microarrays at Detect protein-protein interaktioner

Realtidsmålinger membranprotein: Receptor Interactions Brug overfladeplasmonresonans (SPR)

Identifikation af protein-protein-interaktion websteder anvendelse af peptid Arrays

Read Article