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Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions
Journal JoVE
Biologie
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Journal JoVE Biologie
Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions
DOI:

14:43 min

August 27, 2014

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Chapitres

  • 00:05Titre
  • 02:39Optical Tweezers Setup with Nanometer Stability
  • 04:40Microfluidics
  • 08:36Combined Optical Trapping and Single-molecule Fluorescence Imaging Experiments
  • 11:50Results: Detection of Single Protein Molecules Interacting with a Single DNA Molecule
  • 12:56Conclusion

Summary

Traduction automatique

Here we describe the instrumentation and methods for detecting single fluorescently-labeled protein molecules interacting with a single DNA molecule suspended between two optically trapped microspheres.

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