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Polysomprofiler Fraktionering og analyse af pattedyr Translatomes på en genom-plan
Journal JoVE
Biologie
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Journal JoVE Biologie
Polysome Fractionation and Analysis of Mammalian Translatomes on a Genome-wide Scale

Polysomprofiler Fraktionering og analyse af pattedyr Translatomes på en genom-plan

DOI:
10.3791/51455-v

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10:56 min

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May 17, 2014

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, , , , , ,
1Lady Davis Institute and Department of Oncology,McGill University, 2Department of Oncology-Pathology,Karolinska Institutet, 3Goodman Cancer Centre and Department of Biochemistry,McGill University

Chapitres

  • 00:05Titre
  • 01:54Preparation of Sucrose Gradient
  • 03:38Isolation and Sedimentation of Polysomes
  • 06:59Polysome Fractionation and RNA Extraction
  • 09:22Results: Analytics of Translational Activity in MCF7 Cells Using Polysome Fractionation
  • 10:24Conclusion

Summary

Traduction automatique

Traduction automatique

Ribosomer spiller en central rolle i proteinsyntesen. Polyribosome (polysom) fraktionering af saccharosedensitetsgradientcentrifugering giver mulighed for direkte bestemmelse af oversættelse effektiviteten af ​​individuelle mRNAer på en genom-plan. Desuden kan denne metode anvendes til biokemisk analyse af ribosom-og polysom-associerede faktorer såsom chaperoner og signalstoffer.

Tags

Keywords: MRNA TranslationTranslatomeRibosomePolysomeSucrose Density Gradient CentrifugationAnota AlgorithmGenome-wide AnalysisProtein FoldingPost-translational Modifications

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