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Método in vitro para observar interações E-selectina mediadas entre próstata células tumorais circulantes derivadas de pacientes e células endoteliais humanas
Journal JoVE
Médecine
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Journal JoVE Médecine
In vitro Method to Observe E-selectin-mediated Interactions Between Prostate Circulating Tumor Cells Derived From Patients and Human Endothelial Cells
DOI:

11:56 min

May 15, 2014

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Chapitres

  • 00:05Titre
  • 01:50Culturing HUVECs on Microslides for Observing Circulating Tumor Cell (CTC)-endothelial Interactions
  • 04:04Preparation of Flow Chamber Assembly for Overnight HUVEC Culture on a Microslide
  • 05:48Preparation of Anti-PSMA Labeled Prostate Cancer Cells
  • 07:05Preparation of Microscope, Syringe Pump and Flow Chamber Assembly
  • 09:11Results: Analysis of Interactions Between Labeled MDA Prostate Cancer Cells and Endothelial Cells
  • 11:11Conclusion

Summary

Traduction automatique

O nosso relatório descreve um método único para visualizar e analisar as interações CTC / CE no câncer de próstata em condições de fluxo fisiológicos.

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