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Forbigående ekspression af proteiner ved Hydrodynamisk Gene Delivery i mus
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Journal JoVE Biologie
Transient Expression of Proteins by Hydrodynamic Gene Delivery in Mice

Forbigående ekspression af proteiner ved Hydrodynamisk Gene Delivery i mus

DOI:
10.3791/51481-v

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12:54 min

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May 05, 2014

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,
1Department of Biological Sciences,Hunter College, CUNY

Chapitres

  • 00:05Titre
  • 01:23Weighing Mice and Preparing Syringes
  • 03:38Hydrodynamic DNA Delivery
  • 08:18Large-volume Tail Vein Injection using an Alternative Hand Position
  • 09:41Representative Analysis of Transgene Expression
  • 11:50Conclusion

Summary

Traduction automatique

Traduction automatique

In vivo transfektion af nøgent DNA ved hydrodynamisk genlevering indføres gener i væv i et dyr med minimal inflammatorisk reaktion. Genereres tilstrækkelige mængder af gen-produkt, således at gen-funktion og regulering samt proteiners struktur og funktion kan analyseres.

Tags

Keywords: Hydrodynamic Gene DeliveryIn Vivo Gene ExpressionTransgene DeliveryPlasmid DNATransient Protein ExpressionApolipoprotein L-IHaptoglobin-related ProteinRodent Models

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