Journal
/
Médecine
/
Modeling Spontaneous Metastatic Renal Cell Carcinoma (mRCC) in Mice Following Nephrectomy
Journal JoVE
Médecine
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Médecine
Modeling Spontaneous Metastatic Renal Cell Carcinoma (mRCC) in Mice Following Nephrectomy

Modeling Spontaneous Metastatic Renal Cell Carcinoma (mRCC) in Mice Following Nephrectomy

DOI:
10.3791/51485-v

•

11:27 min

•

April 29, 2014

•

, , , ,
1Genitourinary Section, Department of Medicine,Roswell Park Cancer Institute, 2Biological Sciences Platform,Sunnybrook Research Institute

Chapitres

  • 00:05Titre
  • 02:13Orthotopic Kidney Tumor Implantation
  • 05:37Nephrectomy/Primary Tumor Removal
  • 06:51Monitoring Metastatic Progression and Localization at Endpoint
  • 09:47Results: Assessment of Metastasis Location and Severity
  • 10:19Conclusion

Summary

Traduction automatique

Traduction automatique

Models of spontaneous metastatic renal cell carcinoma (RCC) disease progression can be used for evaluating treatments in a clinically relevant setting. This protocol demonstrates different procedures for orthotopic kidney tumor cell implantation, proper nephrectomy, and finally outlines a necropsy guide for visual and bioluminescent scoring of metastatic burden and localization.

Tags

Metastatic Renal Cell CarcinomaMRCCMouse ModelNephrectomyOrthotopic Tumor ImplantationSyngeneic ModelBioluminescent ImagingMetastatic Disease ProgressionTherapeutic Testing

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

Single Port Donor Nephrectomy

Modeling Stroke in Mice – Middle Cerebral Artery Occlusion with the Filament Model

Mammary Transplantation of Stromal Cells and Carcinoma Cells in C57BL/6J Mice

Multi-photon Imaging of Tumor Cell Invasion in an Orthotopic Mouse Model of Oral Squamous Cell Carcinoma

Modeling Intracerebral Hemorrhage in Mice: Injection of Autologous Blood or Bacterial Collagenase

Generation of Subcutaneous and Intrahepatic Human Hepatocellular Carcinoma Xenografts in Immunodeficient Mice

Induction of Invasive Transitional Cell Bladder Carcinoma in Immune Intact Human MUC1 Transgenic Mice: A Model for Immunotherapy Development

Initiation of Metastatic Breast Carcinoma by Targeting of the Ductal Epithelium with Adenovirus-Cre: A Novel Transgenic Mouse Model of Breast Cancer

Modeling Stroke in Mice: Permanent Coagulation of the Distal Middle Cerebral Artery

Modeling Chemotherapy Resistant Leukemia In Vitro

Read Article