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Method for Obtaining Primary Ovarian Cancer Cells From Solid Specimens
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Médecine
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Journal JoVE Médecine
Method for Obtaining Primary Ovarian Cancer Cells From Solid Specimens

Method for Obtaining Primary Ovarian Cancer Cells From Solid Specimens

DOI:
10.3791/51581-v

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05:58 min

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February 04, 2014

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, , , , , , ,
1Department of Obstetrics, Gynecology, and Women’s Heath,University of Minnesota, 2Department of Obstetrics and Gynecology,Maricopa Medical Center and St Josephs Hospital and Medical Center, 3Masonic Cancer Center,University of Minnesota

Chapitres

  • 00:05Titre
  • 01:34Reagent Setup
  • 02:00Tissue Processing
  • 04:45Results: Cell Culture Profile
  • 05:37Conclusion

Summary

Traduction automatique

Traduction automatique

This study describes a detailed method for isolation and characterization of primary ovarian cancer cells from solid clinical specimens. Ovarian cancer clinical specimens are subjected to enzymatic digestion to obtain viable, fibroblast-free epithelial ovarian cancer (EOC) cells highly suitable for downstream applications.

Tags

Keywords: Primary Ovarian Cancer CellsSolid SpecimensCell IsolationEnzymatic DigestionCell CultureDrug ScreeningOvarian Cancer Cell LinesHeterogeneityGenetic AlterationsIn Vitro PassagingClinical SpecimensMetastatic Ovarian Cancer

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