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Immunologie et infection
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High Yield Purification of Plasmodium falciparum Merozoites For Use in Opsonizing Antibody Assays
Journal JoVE
Immunologie et infection
This content is Free Access.
Journal JoVE Immunologie et infection
High Yield Purification of Plasmodium falciparum Merozoites For Use in Opsonizing Antibody Assays

High Yield Purification of Plasmodium falciparum Merozoites For Use in Opsonizing Antibody Assays

DOI:
10.3791/51590-v

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10:38 min

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July 17, 2014

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, ,
1Division of Infection and Immunity,Walter and Eliza Hall Institute of Medical Research, 2Department of Medical Biology,University of Melbourne

Chapitres

  • 00:05Titre
  • 01:34Isolation of P. falciparum Merozoites
  • 04:42Quantifying Merozoite Concentration by Flow Cytometry
  • 06:03Phagocytosis Assay
  • 07:48Results: The Merzoite:THP-1 Ratio Influences the Degree of Phagocytosis Observed
  • 09:38Conclusion

Summary

Traduction automatique

Traduction automatique

Measuring antibody function is key to understanding immunity to Plasmodium falciparum malaria. This method describes the purification of viable merozoites, and measurement of opsonization-dependent phagocytosis by flow cytometry.

Tags

Keywords: Plasmodium FalciparumMerozoitesMalaria VaccineOpsonizing AntibodiesPhagocytosisTHP-1 CellsE64 Protease InhibitorHigh-yield PurificationAssay Development

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