Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Chapitres
- 00:05Titre
- 01:12Bile Duct Isolation
- 02:59Embedding Tissues into Thick Collagen Gels
- 04:19Isolating Primary Cholangiocytes
- 07:05Characterizing Cultured Cholangiocytes
- 07:24Conclusion
A technique to isolate cholangiocytes from the extrahepatic bile ducts of neonatal mice is described. The ducts are meticulously dissected, and then cells are isolated by outgrowth in thick collagen gels. This method provides a useful tool for studying extrahepatic bile duct development and pathology.
