Journal

The Use of Fluorescent Target Arrays for Assessment of T Cell Responses In vivo

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Immunologie et infection

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Journal JoVE Immunologie et infection

The Use of Fluorescent Target Arrays for Assessment of T Cell Responses In vivo

The Use of Fluorescent Target Arrays for Assessment of T Cell Responses In vivo

DOI:

10.3791/51627-v

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12:09 min

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June 19, 2014

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Benjamin J. C. Quah, Danushka K. Wijesundara, Charani Ranasinghe, Christopher R. Parish

¹Department of Immunology, John Curtin School of Medical Research,Australian National University

Chapitres

00:05Titre
01:39Fluorescent Target Array Preparation: CTV and CFSE Labeling
04:54FTA Preparation: Peptide Pulsing and Cell Washing
07:50FTA Preparation: CPD Cell Labeling
09:43FTA Assay in Response to VV/HIV Epitopes
11:45Conclusion

Summary

Traduction automatique

The ability to monitor T cell responses in detail in vivo is important for the development of our understanding of the immune response. Here we describe the use of fluorescent target arrays (FTAs) in an in vivo T cell assay that assesses >250 parameters simultaneously by flow cytometry.

The Use of Fluorescent Target Arrays for Assessment of T Cell Responses In vivo

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Read Article

The Use of Fluorescent Target Arrays for Assessment of T Cell Responses In vivo

•

•

•

Chapitres

Summary

Traduction automatique

Tags

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

Retroviral Transduction of T-cell Receptors in Mouse T-cells

Preparation and Use of HIV-1 Infected Primary CD4+ T-Cells as Target Cells in Natural Killer Cell Cytotoxic Assays

Introducing Shear Stress in the Study of Bacterial Adhesion

Induction of Graft-versus-host Disease and In Vivo T Cell Monitoring Using an MHC-matched Murine Model

Transplantation of Tail Skin to Study Allogeneic CD4 T Cell Responses in Mice

In Vitro and In Vivo Assessment of T, B and Myeloid Cells Suppressive Activity and Humoral Responses from Transplant Recipients

In Vivo Assay for Detection of Antigen-specific T-cell Cytolytic Function Using a Vaccination Model

Isolation of Salmonella typhimurium-containing Phagosomes from Macrophages

A Suction Blister Protocol to Study Human T-cell Recall Responses In Vivo

Evaluation of Zika Virus-specific T-cell Responses in Immunoprivileged Organs of Infected Ifnar1-/- Mice

Read Article

Evaluation of Zika Virus-specific T-cell Responses in Immunoprivileged Organs of Infected Ifnar1^-/- Mice