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Studerer DNA Looping av Single-Molecule FRET
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Journal JoVE Biologie
Studying DNA Looping by Single-Molecule FRET

Studerer DNA Looping av Single-Molecule FRET

DOI:
10.3791/51667-v

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11:27 min

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June 28, 2014

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,
1School of Physics,Georgia Institute of Technology

Chapitres

  • 00:05Titre
  • 01:21Double Stranded (ds)DNA Sample Preparation
  • 02:52Flow Cell Preparation
  • 05:04Single-molecule Imaging
  • 06:18Image Processing and Data Analysis
  • 07:48Determining the J Factor
  • 09:44Results: Representative Analysis of DNA Looping
  • 10:52Conclusion

Summary

Traduction automatique

Traduction automatique

Denne studien presenterer en detaljert eksperimentell prosedyre å måle looping dynamikken i dobbel-strandet DNA ved hjelp av single-molekyl fluorescens Resonance Energy Transfer (FRET). Protokollen beskriver også hvordan du kan hente looping sannsynlighetstetthet kalt J-faktor.

Tags

DNA LoopingFRETDNA BendingDNA-protein InteractionsDNA PackagingNucleosomeCyclizationDNA LigaseDNA StructureLooping KineticsPCRFRET PairBiotin LinkerJ FactorIntrinsic Curvature

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