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Microscale Vortex-assisted Electroporator for Sequential Molecular Delivery
Journal JoVE
Bio-ingénierie
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Journal JoVE Bio-ingénierie
Microscale Vortex-assisted Electroporator for Sequential Molecular Delivery
DOI:

10:51 min

August 07, 2014

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Chapitres

  • 00:05Titre
  • 01:48Cell Preparation
  • 03:01Flow Experiments
  • 08:32Results: Macromolecules of Various Sizes and Electrical Charges are Delivered into Living Cells
  • 10:09Conclusion

Summary

Traduction automatique

A microfluidic vortex assisted electroporation platform was developed for sequential delivery of multiple molecules into identical cell populations with precise and independent dosage control. The system’s size based target cell purification step preceding electroporation aided to enhance molecular delivery efficiency and processed cell viability.

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