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A Protocol to Infect Caenorhabditis elegans with Salmonella typhimurium

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Immunologie et infection

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Journal JoVE Immunologie et infection

A Protocol to Infect Caenorhabditis elegans with Salmonella typhimurium

A Protocol to Infect Caenorhabditis elegans with Salmonella typhimurium

DOI:

10.3791/51703-v

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06:50 min

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June 26, 2014

•

Jiuli Zhang, Kailiang Jia

¹Department of Biological Sciences,Florida Atlantic University

Chapitres

00:05Titre
00:53Preparations
02:01Feeding RNAi to Nematodes
03:34Treating C. elegans with Salmonella and Scoring Survival
05:41C. elegans Survival from Salmonella Infection and bec-1
06:25Conclusion

Summary

Traduction automatique

C. elegans has emerged as a new genetic model to study host-pathogen interactions. Here we describe a protocol to infect C. elegans with Salmonella typhimurium coupled with the double-strand RNAi interference technique to examine the role of host genes in defense against Salmonella infection.

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