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RNA Catalyst som reporter for Screening narkotika mot RNA Redigering i trypanosomer
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Journal JoVE Biologie
RNA Catalyst as a Reporter for Screening Drugs against RNA Editing in Trypanosomes

RNA Catalyst som reporter for Screening narkotika mot RNA Redigering i trypanosomer

DOI:
10.3791/51712-v

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09:19 min

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July 22, 2014

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, ,
1Department of Biochemistry,McGill University, 2Institute of Parasitology,McGill University, 3McGill Centre for Bioinformatics,McGill University

Chapitres

  • 00:05Titre
  • 01:34T. brucei Cell Culture
  • 02:22Crude Mitochondria Isolation
  • 03:56Editosome Purification
  • 04:46RNA Preparation
  • 06:13Fluorescence-based RNA Editing Assay
  • 07:55Results: Determining the Assay Quality
  • 08:54Conclusion

Summary

Traduction automatique

Traduction automatique

A highly sensitive ribozyme-based assay, applicable to high-throughput screening of chemicals targeting the unique process of RNA editing in trypanosomatid pathogens, is described in this paper. Inhibitors can be used as tools for hypothesis-driven analysis of the RNA editing process and ultimately as therapeutics.

Tags

RNA EditingTrypanosomesEditosomeFluorescence-based Reporter AssayFRETHigh-throughput ScreeningChemical CompoundsMolecular ProbesIn Vitro RNA Editing

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