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Nanogold Labeling of the Yeast Endosomal System for Ultrastructural Analyses
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Journal JoVE Biologie
Nanogold Labeling of the Yeast Endosomal System for Ultrastructural Analyses

Nanogold Labeling of the Yeast Endosomal System for Ultrastructural Analyses

DOI:
10.3791/51752-v

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09:49 min

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July 14, 2014

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, ,
1Department of Cell Biology,University Medical Center Utrecht

Chapitres

  • 00:05Titre
  • 01:21Spheroplast Preparation
  • 03:07Nanogold Uptake
  • 03:44Fixation and Sectioning
  • 04:42Silver Enhancement for Nanogold Particle Visualization
  • 08:17Results: Yeast Endosomal Compartments are Visualized by Silver-enhanced Nanogold Labeling
  • 09:09Conclusion

Summary

Traduction automatique

Traduction automatique

Yeast, Saccharomyces cerevisiae, has been a key model organism to identify and study genes regulating the biogenesis and functions of the endosomal system. Here we present a detailed protocol for the specific labeling of the endosomal compartments for ultrastructural studies.

Tags

Keywords: Endosomal SystemYeastSaccharomyces CerevisiaeNanogold LabelingUltrastructural AnalysisSpheroplastsSilver EnhancementVesicular TransportCell HomeostasisPathologiesCancerNeurobiological DisordersProtein Localization

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