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Fluorescensimagografi med One-nanometer Nøjagtighed (FIONA)
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Journal JoVE Biologie
Fluorescence Imaging with One-nanometer Accuracy (FIONA)

Fluorescensimagografi med One-nanometer Nøjagtighed (FIONA)

DOI:
10.3791/51774-v

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11:56 min

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September 26, 2014

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, , , , ,
1Department of Physics,University of Illinois at Urbana-Champaign, 2Center for the Physics of Living Cells,University of Illinois at Urbana-Champaign, 3Center for Biophysics and Computational Biology,University of Illinois at Urbana-Champaign

Chapitres

  • 00:05Titre
  • 01:11TIRM Setup
  • 04:40FIONA on Cy3-DNA
  • 06:28Data Analysis for FIONA on Cy3-DNA
  • 07:29Application of FIONA to Quantify Motor Dynamics at Nanometer Scale
  • 09:10Data Analysis and Finding the Step-size of Myosin Walking
  • 09:59Results: Single Fluorophores Are Localized with Nanometer Precision Using FIONA
  • 11:17Conclusion

Summary

Traduction automatique

Traduction automatique

Enkelte fluoroforer kan lokaliseres med nanometer præcision ved hjælp af FIONA. Her er en sammenfatning af FIONA teknik er rapporteret, og hvordan man udfører FIONA eksperimenter beskrives.

Tags

Keywords: Fluorescence ImagingFIONASingle FluorophoresNanometer PrecisionTIRFMCy3-DNAMyosin Va MotorQuantum DotsSol-gelsRabbit Eye Corneas

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