Journal
/
/
3D تتبع المداري في التعديل المجهر ثنائي الفوتون: دراسة تطبيقية على تتبع من بين الخلايا الحويصلات
Journal JoVE
Bio-ingénierie
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Bio-ingénierie
3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles
DOI:

11:28 min

October 01, 2014

, ,

Chapitres

  • 00:05Titre
  • 01:50Sample Preparation
  • 03:19Microscope Configurations
  • 05:05Imaging
  • 07:21Trajectory Analysis
  • 08:40Results: Fast 3D Single Particle Tracking of an Isolated Lysosome Moving Inside the Cell After the Endosome Maturation Process
  • 10:36Conclusion

Summary

Traduction automatique

In this video protocol we track - at high speed and in three dimensions - fluorescently labeled lysosomes within living cells, using the orbital tracking method in a modified two-photon microscope.

Vidéos Connexes

Read Article