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A Novel in vivo Gene Transfer Technique and in vitro Cell Based Assays for the Study of Bone Loss in Musculoskeletal Disorders
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Médecine
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Journal JoVE Médecine
A Novel in vivo Gene Transfer Technique and in vitro Cell Based Assays for the Study of Bone Loss in Musculoskeletal Disorders

A Novel in vivo Gene Transfer Technique and in vitro Cell Based Assays for the Study of Bone Loss in Musculoskeletal Disorders

DOI:
10.3791/51810-v

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11:47 min

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June 08, 2014

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, , , , , , ,
1Division of Rheumatology, Allergy and Clinical Immunology,University of California, Davis, 2Institute for Pediatric Regenerative Medicine,Shriners Hospitals for Children – Northern California, 3Department of Dermatology,University of California, Davis

Chapitres

  • 00:00Titre
  • 00:39Hydrodynamic delivery of RANKL MC DNA
  • 03:04In vitro osteoclast generation from mouse BMM
  • 05:42In vitro osteoclast generation from human PBMC
  • 09:31Résultats

Summary

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Traduction automatique

Differentiation of precursor cells into osteoclasts is regulated by cytokines and growth factors. Here, a novel gene transfer technique for differentiation of osteoclasts in vivo and cell culture protocols for differentiating precursor cells into osteoclasts in vitro as a method to study the effects of cytokines on osteoclastogenesis are described.

Tags

OsteoclastsBone ResorptionMusculoskeletal DisordersIn Vivo Gene TransferIn Vitro Cell-based AssaysCytokinesOsteoclastogenesisOsteoporosisArthritisDrug Discovery

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