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Analysis of Tubular Membrane Networks in Cardiac Myocytes from Atria and Ventricles
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Chapitres
- 00:05Titre
- 01:46Isolation of Atrial and Ventricular Myocytes from the Mouse Heart
- 04:09Imaging of Transverse-axial Tubule System (TATS) Membrane Structures in Living Myocytes
- 05:41Analysis of the TATS Membrane Network and its Components
- 08:23Results: Representative TATS Component Orientations for Ventricular and Atrial Myocytes
- 09:26Conclusion
In cardiac myocytes, tubular membrane structures form intracellular networks. We describe optimized protocols for i) isolation of myocytes from mouse heart including quality control, ii) live cell staining for state-of-the-art fluorescence microscopy, and iii) direct image analysis to quantify the component complexity and the plasticity of intracellular membrane networks.
