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管式膜网络在心肌细胞的心房和心室分析

Journal JoVE
Bio-ingénierie

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Journal JoVE Bio-ingénierie

Analysis of Tubular Membrane Networks in Cardiac Myocytes from Atria and Ventricles

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管式膜网络在心肌细胞的心房和心室分析

DOI:

10.3791/51823-v

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10:30 min

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October 15, 2014

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Eva Wagner*^1,2,3, Sören Brandenburg*^1,2, Tobias Kohl², Stephan E. Lehnart^2,3,4

¹Heart Research Center Goettingen, ²Clinic of Cardiology & Pulmonology,University Medical Center Goettingen, ³German Center for Cardiovascular Research (DZHK) partner site Goettingen, ⁴BioMET, Center for Biomedical Engineering & Technology,University of Maryland School of Medicine

Chapitres

00:05Titre
01:46Isolation of Atrial and Ventricular Myocytes from the Mouse Heart
04:09Imaging of Transverse-axial Tubule System (TATS) Membrane Structures in Living Myocytes
05:41Analysis of the TATS Membrane Network and its Components
08:23Results: Representative TATS Component Orientations for Ventricular and Atrial Myocytes
09:26Conclusion

Summary

Traduction automatique

在心肌细胞，管式膜结构形成细胞内的网络。我们描述了优化方案从小鼠心脏肌细胞I）的隔离，包括质量控制，二）活细胞染色国家的最先进的荧光显微镜，及iii）直接图像分析来量化成分的复杂性和膜细胞的可塑性网络。

Tags

Cardiac Myocytes Transverse-axial Tubule System (TATS)Atrial Myocytes Ventricular Myocytes Sarcoendoplasmic Reticulum Calcium Release Units Membrane Networks Fluorescence Imaging Confocal Microscopy Superresolution Imaging Image Analysis Quantitative Analysis Physiological Adaptation Disease Changes

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