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在植物和绿藻的捕光配合体外重组
Journal JoVE
Biologie
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Journal JoVE Biologie
In Vitro Reconstitution of Light-harvesting Complexes of Plants and Green Algae
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在植物和绿藻的捕光配合体外重组

DOI:
10.3791/51852-v

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11:55 min

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October 10, 2014

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, ,
1Department of Physics and Astronomy,VU University Amsterdam

Chapitres

  • 00:05Titre
  • 01:24Total Pigment Extraction from Spinach Leaves
  • 04:52Reconstitution
  • 07:37Nickel Column Purification
  • 08:18Sucrose Gradient Centrifugation
  • 09:21Results: Reconstituted LHCs Have the Same Properties as Native LHCs
  • 11:33Conclusion

Summary

Traduction automatique

Traduction automatique

This protocol details the reconstitution of light-harvesting complexes in vitro. These integral membrane proteins coordinate chlorophylls and carotenoids and are responsible for harvesting light in higher plants and green algae.

Tags

Keywords: In Vitro ReconstitutionLight-harvesting ComplexesPlantsGreen AlgaeChlorophyllsCarotenoidsThylakoid MembraneChloroplastRecombinant ProteinsPigment CompositionProtein MutationProtein FoldingProtein-protein Interaction

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