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Actividad telomerasa en las diversas regiones del cerebro de ratón: La telomerasa no radiactivo Repetir Protocolo de Amplificación (TRAP) Ensayo
Journal JoVE
Neurosciences
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Journal JoVE Neurosciences
Telomerase Activity in the Various Regions of Mouse Brain: Non-Radioactive Telomerase Repeat Amplification Protocol (TRAP) Assay
DOI:

10:14 min

September 02, 2014

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Chapitres

  • 00:05Titre
  • 01:35Mouse Brain Protein Extraction
  • 03:25Telomeric Repeat Amplification Protocol (TRAP)
  • 06:12Agarose Mini-gel Electrophoresis and Gel Staining
  • 08:16Results: The Modified TRAP Assay is More Sensitive than the Traditional Assay in Detecting Telomerase Activity
  • 09:45Conclusion

Summary

Traduction automatique

Telomerase is expressed in the neonatal brain and also in distinct regions of the adult brain. We present a non-toxic time saving TRAP assay for the analysis of telomerase activity in various regions of the mouse brain and detection of differences in telomerase activity between male and female mouse brains.

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