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Chapitres
- 00:05Titre
- 01:12Dissection of Pupal Testes
- 03:40Dissection of Male Germ-line Cysts and Live Imaging
- 04:58Pharmacological Assay with Intact Pupal Testes
- 06:30Results: Dynamic Analysis of Drosophila Testes
- 08:08Conclusion
This protocol describes the dissection and cultivation of intact testes and germ-line cysts from Drosophila melanogaster pupae. This method allows microscopic observation of spermatogenesis ex vivo. Furthermore, we describe a pharmacological assay of the effect of inhibitors on specific stages of germ-cell development in pupal testes.
