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JoVE Journal Bioengineering
A Novel Method for Localizing Reporter Fluorescent Beads Near the Cell Culture Surface for Traction Force Microscopy
 

A Novel Method for Localizing Reporter Fluorescent Beads Near the Cell Culture Surface for Traction Force Microscopy

Article DOI: 10.3791/51873-v • 13:30 min • September 16th, 2014
September 16th, 2014 •
1, 1, 1
1Department of Mechanical Science and Engineering, University of Illinois at Urbana-Champaign

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Traditional techniques for fabricating polyacrylamide (PA) gels containing fluorescent probes involve sandwiching a gel between an adherent surface and a glass slide. Here, we show that coating this slide with poly-D-lysine (PDL) and fluorescent probes localizes the probes to within 1.6 µm from the gel surface.

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Keywords: Traction Force Microscopy PA Gels Fluorescent Beads Cell Traction Forces Surface Deformation Elasticity Theory Cell Adhesion Extracellular Matrix Surface Displacement Bead Localization Poly-D-lysine Gel Fabrication
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