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Chapitres
- 00:05Titre
- 01:07Preparation of the Producer Strain
- 02:13Overlay Assay
- 04:25Results: Representative Overlay Outcomes
- 05:33Conclusion
Competition in Streptococcus pneumoniae is mediated by bacteriocins, small antimicrobial peptides with inhibitory activity towards pneumococcus and other related species. Here we describe an optimized bacterial overlay assay that allows for the characterization of bacteriocin activity and inhibitory spectrum, bacteriocin-specific immunity, and detection of secreted quorum sensing peptides.
