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Использование наложения Пробирной для Качественно Измерьте бактериальной продукции и чувствительность к пневмококковой Бактериоцины
Journal JoVE
Immunologie et infection
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Journal JoVE Immunologie et infection
Using the Overlay Assay to Qualitatively Measure Bacterial Production of and Sensitivity to Pneumococcal Bacteriocins
DOI:

06:05 min

September 30, 2014

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Chapitres

  • 00:05Titre
  • 01:07Preparation of the Producer Strain
  • 02:13Overlay Assay
  • 04:25Results: Representative Overlay Outcomes
  • 05:33Conclusion

Summary

Traduction automatique

Competition in Streptococcus pneumoniae is mediated by bacteriocins, small antimicrobial peptides with inhibitory activity towards pneumococcus and other related species.  Here we describe an optimized bacterial overlay assay that allows for the characterization of bacteriocin activity and inhibitory spectrum, bacteriocin-specific immunity, and detection of secreted quorum sensing peptides.

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