Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Chapitres
- 00:05Titre
- 01:21Preparation of Plasmid DNA, Needles, and Injection Dishes
- 02:56Preparing Embryos for Injection
- 04:24Microinjection of Plasmid DNA
- 06:02Culturing and Hatching Embryos
- 07:19Results: Establishing Transgenic Hydra Lines
- 08:46Conclusion
Stably transgenic Hydra are made by microinjection of plasmid DNA into embryos followed by random genomic integration and asexual propagation to establish a uniform line. Transgenic Hydra are used to track cell movements, overexpress genes, study promoter function, or knock down gene expression using RNAi.
