Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Chapitres
- 00:00Titre
- 02:22Isolation of Cervical Mononuclear Cells (CMCs)
- 05:11CMC Surface Staining and Flow Cytometry
- 08:06Collection and Preparation of Cervical-Vaginal Lavage (CVL)
- 08:36Data Acquisition and Analysis
- 11:56Conclusion
The use of cytobrush sampling to collect lymphocytes and monocytes from the endocervix is a minimally invasive technique that provides samples for analysis of female genital tract immunity. In this protocol, we describe the collection of cytobrush samples and immune cell isolation for flow cytometry assays.
