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Ikke-enzymatisk, Serumfrit Tissue Culture of Pre-invasive brystlæsioner til spontane generation af Mammospheres
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Biologie
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Journal JoVE Biologie
Non-enzymatic, Serum-free Tissue Culture of Pre-invasive Breast Lesions for Spontaneous Generation of Mammospheres

Ikke-enzymatisk, Serumfrit Tissue Culture of Pre-invasive brystlæsioner til spontane generation af Mammospheres

DOI:
10.3791/51926-v

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09:49 min

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November 08, 2014

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, ,
1Center for Applied Proteomics and Molecular Medicine,George Mason University, 2Virginia Surgery Associates

Chapitres

  • 00:05Titre
  • 02:03Tissue Acquisition and Grossing
  • 04:22Maintenance of Established Organoid/Epithelial Cell Colonies
  • 06:43Results: Representative Mammosphere Imaging
  • 08:51Conclusion

Summary

Traduction automatique

Traduction automatique

Primær cellekultur anvendelse af intakte væv organoids tilvejebringer en model, der efterligner den flercellede in vivo mikromiljø. Vi udviklede en serum-frit primær brystepitel vævskultur model, der foreviger blandede cellekultur slægter og udstillinger differentierede morfologi, uden enzymatisk vævssprængning. Breast organoids forbliver levedygtige i> 6 måneder.

Tags

Keywords: Non-enzymaticSerum-freePre-invasive Breast LesionsSpontaneous GenerationMammospheresBreast Ductal Carcinoma In Situ (DCIS)Progenitor CellsInvasionOrganoidsEpithelial CellsFibroblastsCytogenetically AbnormalInvasive Progenitor Cells

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