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Uma Experimental e Bioinformática Protocolo de Análises de RNA-seq de fotoperiódicas diapausa no Tigre Asiático Mosquito,<em> Aedes albopictus</em
Journal JoVE
Biologie
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Journal JoVE Biologie
An Experimental and Bioinformatics Protocol for RNA-seq Analyses of Photoperiodic Diapause in the Asian Tiger Mosquito, Aedes albopictus
DOI:

12:10 min

November 30, 2014

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Chapitres

  • 00:05Titre
  • 01:55Larval Rearing of Two A. albopictus Groups to Adulthood
  • 04:25Measuring Diapause Incidence
  • 07:06RNA Extraction from Eggs
  • 08:56Representative RNA Sequencing Analysis of A. albopictus Extracts
  • 11:07Conclusion

Summary

Traduction automatique

RNA-Seq analyses are becoming increasingly important for identifying the molecular underpinnings of adaptive traits in non-model organisms. Here, a protocol to identify differentially expressed genes between diapause and non-diapause Aedes albopictus mosquitoes is described, from mosquito rearing, to RNA sequencing and bioinformatics analyses of RNA-Seq data.

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