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Utilizing Custom-designed Galvanotaxis Chambers to Study Directional Migration of Prostate Cells
Journal JoVE
Biologie
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Journal JoVE Biologie
Utilizing Custom-designed Galvanotaxis Chambers to Study Directional Migration of Prostate Cells
DOI:

08:45 min

December 07, 2014

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Chapitres

  • 00:05Titre
  • 01:11Assembling the Galvanotaxis Chamber and Agar Bridges
  • 05:37Time-lapse Imaging
  • 06:58Quantification of Galvanotaxis
  • 07:41Results: Representative Galvanotaxis of Prostate Cells
  • 08:24Conclusion

Summary

Traduction automatique

We present a method to apply a physiological electric field to migrating, immortalized prostate cells in a custom-made galvanotaxis chamber. Using this method, we demonstrate that 2 lines of non-tumorigenic prostate cells demonstrate different degrees of migration directionality in the field.

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