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Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope
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Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

DOI:
10.3791/51993-v

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08:53 min

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August 15, 2014

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, ,
1Institute of Applied Research,Aalen University

Chapitres

  • 00:05Titre
  • 02:03Cell Spheroids Incubated with a Chemotherapeutic Drug
  • 02:55Light Sheet Adjustment
  • 04:09Cell Spheroid Application and Microscope Feed Synchronization
  • 05:31Measurement in Dynamic Liquid Environment and Data Acquisition and Analysis
  • 07:00Results: Imaging of Cell Spheroids Incubated with the Cytostatic Drug Doxorubicin
  • 08:07Conclusion

Summary

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A module for single plane illumination microscopy (SPIM) is described which is easily adapted to an inverted wide-field microscope and optimized for 3-dimensional cell cultures. The sample is located within a rectangular capillary, and via a microfluidic system fluorescent dyes, pharmaceutical agents or drugs can be applied in small quantities.

Tags

Keywords: Single Plane IlluminationLight Sheet MicroscopySPIM3D Cell CultureMulti-cellular Tumor SpheroidsMicro-capillaryWide-field MicroscopeFluorescence DetectionMicrofluidicsDoxorubicinGlutathione SensorCell Necrosis

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