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Chapitres
- 00:05Titre
- 01:58Virus Infection of Embryoid Body (EB) Cells
- 04:02Differentiating Viral Infected EB Cells in Hanging Drops
- 05:47Preparing Single Cell Suspensions for Flow Cytometer Analysis
- 07:02Flow Cytometry Analysis for Hematopoietic Progenitors
- 08:48Results: Analysis of Retroviral Infected EBs and Hematopoietic Progenitor Cell Generation
- 11:00Conclusion
Manipulating temporal gene expression in differentiating embryonic stem cells (ESCs) can be achieved using inducible gene systems. However, generation of these cell lines is costly and time consuming. This protocol achieves rapid expression of a transgene in differentiating ES-derived cells and subsequent analysis of downstream hematopoietic differentiation.
