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Real Time Analysis of Metabolic Profile in Ex Vivo Mouse Intestinal Crypt Organoid Cultures
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Journal JoVE Biologie
Real Time Analysis of Metabolic Profile in Ex Vivo Mouse Intestinal Crypt Organoid Cultures

Real Time Analysis of Metabolic Profile in Ex Vivo Mouse Intestinal Crypt Organoid Cultures

DOI:
10.3791/52026-v

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08:53 min

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November 03, 2014

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,
1Department of Medicine,Albert Einstein College of Medicine, 2Department of Cell Biology,Albert Einstein College of Medicine

Chapitres

  • 00:05Titre
  • 01:42Crypt Isolation and Culture
  • 06:17Extracellular Flux Assay
  • 07:36Results: Metabolism Assay Using Organoids Derived from 8 month old C57BL/6 Mice
  • 08:20Conclusion

Summary

Traduction automatique

Traduction automatique

Small intestinal crypt organoids cultured ex vivo provide a tissue culture system that recapitulates growth of crypts dependent on stem cells and their niche. We established a method to assay the metabolic profile in real time in primary mouse crypt organoids. We found organoids maintain physiological properties defined by their source.

Tags

Keywords: Intestinal Crypt OrganoidMouse IntestineMetabolic ProfileOxygen ConsumptionGlycolytic RateATP ProductionRespiratory CapacityReal-time AnalysisCrypt MicroenvironmentIntestinal Epithelial CellsAdult Stem CellsProgenitor CellsPaneth CellsStromal CellsImmune Cells

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